Illuminating Gene Expression with Single-Molecule Precision
The Li Lab seeks a molecular understanding of how cells control the spatial organization of gene expression. We use advanced single-molecule imaging techniques to study the precise localization of mRNAs and proteins, and to understand how their mislocalization can lead to disease.
For instance, in yeast, approximately 20% of all the mRNAs are targeted to the surfaces of the endoplasmic reticulum (ER) and mitochondria, where they are translated to support organelle biogenesis and maintenance. In neurons, certain mRNAs are transported to synapses, where they play essential roles in synaptic activity and learning. Dysregulated mRNA localization in neurons is associated with neurological disorders, including fragile X syndrome (FXS) and amyotrophic lateral sclerosis (ALS).
We aim to uncover the mechanisms that regulate the spatial organization of gene expression, and determine the consequences of its dysregulation.